Human normal colorectal mucosal cells
(Human normal colorectal mucosal cells)
[Cell number]
[Cell abbreviation]
Cells
[Cell name]
(Human normal colorectal mucosal cells)
[Background information]
See cell instructions
[Cell Source]
The main source of cells, human normal colorectal mucosal cells
, and a few well-known domestic and foreign universities established departments
[Generations]
[ Specifications]
Resuscitation culture bottle (one bottle) or cryopreservation tube (two)
[Number of cells]
*
[Biology Safety level]
[Organism]
Human
[Tissue source]
Colorectum
[Cell morphology]
Epithelial cells
[Cell characteristics]
Adhesion
[Cell viability]
)
[ Cell detection]
The cells do not contain mycoplasma, bacteria, yeast and fungi
[Culture conditions]
See the cell instructions for details
[Passaging method]
It is recommended to change the medium once every two days
[Freezing conditions]
See the cell instructions for details
[Main literature]
Please check for details Related published articles
Operation procedures and precautions after receipt
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum Transfer the complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, the remaining adherent cells in the original culture flask are replaced with complete medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
If the adherent cells grow unevenly and grow into islands, the cells can be digested , resuspend and disperse the cells, add fresh culture medium for culture
Routine cell culture and passage process (please strictly follow aseptic operation)
Aspirate the culture medium in the original culture bottle, and rinse both cells with buffer. times, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under the microscope, shrink the edge of the cells, and normal human colorectal mucosal cells
when they loosen their adhesion to the wall (It is not recommended to digest until the cells are floating.) Remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle , add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), and repeat the item after the cell density reaches % Operation or cryopreservation
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Human normal colorectal mucosal cells
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Human normal colorectal mucosal cells
, the company professionally provides The American cell bank sources normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, Cryopreservation conditions and other recovery and cryopreservation instructions information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
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, Company Specializing in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, Tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
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FHC human normal colorectal mucosal cells