Human cholangiocarcinoma cells
(Human cholangiocarcinoma cells)
[Cell number]
[Cell abbreviation]
Cells
[Cell name]
(Human cholangiocarcinoma cells)
[Background information]
See cell instructions
[Cell source]
The cells are mainly derived from human cholangiocarcinoma cells
and from a few well-known universities at home and abroad
[Generations]
[Specifications]
Resuscitation culture bottles ( One bottle) or cryopreserved vials (two)
[Number of cells]
*
[Biosafety level]
[Organism]
Human
[Tissue source]
Bile duct disease cancer
[Cell morphology]
Epithelioid
【Cell Characteristics】
Adhesion
【Cell Viability】
)
【Cell Detection】
Cells are not Contains, mycoplasma, bacteria, yeast and fungi
[Culture conditions]
See cell instructions for details
[Passaging method]
It is recommended to change the medium every two days Once
[Freezing conditions]
For details, please refer to the cell instructions
[Main literature]
Please refer to relevant published articles for details
After receipt Operating procedures and precautions
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to a new culture dish. / bottle continues to be cultured for another day while the remaining adherent cells in the original culture bottle are replaced with complete culture medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
. If the adherent cells grow unevenly and grow into islands, human cholangiocarcinoma cells
br/>The cells can be digested, resuspended and dispersed, and fresh medium added for culture
Routine cell culture and passage procedures (please strictly follow aseptic operation)
Aspirate the culture in the original culture bottle base, rinse the cells twice with buffer, and add. % trypsin to digest cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under a microscope. When the edges of the cells shrink and the adhesion becomes loose (it is not recommended to digest until the cells float), remove the trypsin and add it completely. culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, add appropriate complete culture medium, and stir the cell suspension. Evenly, culture in the incubator
, pay attention to the changes in the culture medium value, change the medium regularly (weekly), repeat the operation or freeze after the cell density reaches %
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, Company Specializing in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, Tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture, so that your experiments will be hassle-free
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Human cholangiocarcinoma cells
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, Recovery and cryopreservation instructions for diseases, tissues, cryopreservation conditions, etc. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
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, The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, and diseases. , tissue, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, Recovery and cryopreservation instructions for diseases, tissues, cryopreservation conditions, etc. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, and systems. , diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphologies, culture conditions, Application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, types, morphologies, and culture conditions , applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
QBC939 human cholangiocarcinoma cell