Human peritoneal mesothelial cells
(Human peritoneal mesothelial cells)
[Cell number]
[Cell abbreviation]
Cells
< br/>[Cell name]
(Human peritoneal mesothelial cells)
[Background information]
See cell instructions
[Cell source] < br/>The cells are mainly derived from human peritoneal mesothelial cells
and from a few well-known universities at home and abroad
[Generations]
[Specifications]
Recovery culture bottle (one bottle) or cryopreservation vial (two)
[Number of cells]
*
[Biosafety level]
【Organism】
Human
【Tissue source】
Mesothelium
【Cell morphology】
Epithelioid
【Cell Characteristics】
Adhesion
【Cell Viability】
)
【Cell Detection】
Cells do not contain mycoplasma, bacteria, yeast and fungi
[Culture conditions]
See cell instructions for details
[Passaging method]
Two recommended Change the medium once a day
[Freezing conditions]
See cell instructions for details
[Main literature]
Please refer to relevant published articles for details
Operation procedures and precautions after receipt
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to new cells. The culture dish/bottle continues to be cultured for another day while the remaining adherent cells in the original culture bottle are replaced with complete culture medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , human peritoneal mesothelial cells
According to the cell growth density, trypsin digestion may be considered and then transferred to a new culture bottle to continue culturing
. If the adherent cells grow unevenly and are unevenly distributed, they will become To grow in an island shape, the cells can be digested, resuspended and dispersed, and then added with fresh medium for culture
Routine cell culture and passage procedures (please strictly follow aseptic operation)
Aspirate out the original culture bottle Rinse the cells twice with culture medium and buffer, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it at)
Observe the digestion situation under the microscope, and when the edges of the cells shrink and the adhesion loosens ( It is not recommended to digest until the cells are floating.) Remove trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle , add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in culture medium value, change the medium regularly (weekly), and repeat the operation after the cell density reaches % Or cryopreservation
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. Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell lines, organisms, growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any worries
Human peritoneal mesothelial cells
.
, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells We provide comprehensive technical support so that your experiments will be trouble-free
. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells We provide all-round technical support so that your experiments will be hassle-free
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells Cultivation provides comprehensive technical support so that your experiments will be trouble-free
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. All-round technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, Source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that Your experiments will be worry-free
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, and growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any trouble
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, and provides cell line organisms and growth characteristics , source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture. Let your experiments be trouble-free
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, and provides cell line organisms, growth characteristics, and sources. , organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to Experiments are no longer troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, sources, and organs , type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to experiment again. No hassle
Human peritoneal mesothelial cells
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. Provide cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide Comprehensive technical support for cell culture, allowing you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture All-round technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support allows you to experiment without any trouble
HMrSV5 human peritoneal mesothelial cells