/Immortalized human brain microvascular endothelial cells
/(Immortalized human brain microvascular endothelial cells)
[Cell number]
[Cell abbreviation]
/cell
[Cell name]
/(Immortalized human brain microvascular endothelial cells)
[Background information]
See cell instructions
[Cell source]
The main source of cells is: Immortalized human brain microvascular endothelial cells
, and a few well-known universities at home and abroad established departments
[Generations]
[Specifications]
Resuscitation culture bottle (one bottle) or cryopreservation tube (two)
[Number of cells]
*< br/>
[Biological safety level]
[Organism]
Human
[Tissue source]
Brain tissue blood-brain barrier
[Cell morphology]
Endothelium
[Cell characteristics]
Adhesion
[Cell viability]
)
[Cell detection]
The cells do not contain mycoplasma, bacteria, yeast and fungi
[Culture conditions]
See the cell instructions for details< br/>
[Passaging method]
It is recommended to change the medium once every two days
[Freezing conditions]
See the cell instructions for details
[Main literature]
Please refer to relevant published articles for details
Operation procedures and precautions after receipt
If the cells are adherent cells and are in a suspended or partially suspended state when received, Please centrifuge the suspended cells immediately, add % serum complete medium to a new culture dish/bottle and continue culturing for another day. At the same time, replace the remaining adherent cells in the original culture bottle with % serum complete medium for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly/immortalized human brain microvascular endothelial cells
Appropriately increase the serum concentration (the maximum cannot exceed %), or according to the cell growth density, consider trypsin digestion and then transfer to a new culture bottle to continue culturing
If the adherent cells grow unevenly and are unevenly distributed, All, growing in an island shape, the cells can be digested, resuspended and dispersed, and added with fresh culture medium for culture
Routine cell culture and passage process (please strictly follow aseptic operation)
Aspirate the original culture Rinse the cells twice with culture medium and buffer in the bottle, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it within)
Observe the digestion situation under the microscope, shrink the edges of the cells, and adhere to the wall When loosening (it is not recommended to digest until the cells are floating), remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, and transfer part of the cell suspension to a new culture dish. / bottle, add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, and change the medium regularly (weekly) until the cell density reaches % Repeated operations or cryopreservation
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be trouble-free
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. The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells A full range of technical support for culture, allowing you to experiment without any worries
/ Immortalized human brain microvascular endothelial cells
[
, the company specializes in providing normal cell lines derived from American cell banks, Tumor cell lines and cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions, etc. recovery and cryopreservation Instructions for information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines and tumor cells from American cell banks. lines, cancer cell lines (more than 3,000 types), providing information on cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. , we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be trouble-free
The company specializes in providing normal cell lines, tumor cell lines, Cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information, we It has rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be trouble-free
The company specializes in providing normal cell lines and tumor cells from American cell banks. lines, cancer cell lines (more than 3,000 types), providing information on cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. , we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines and tumor cells derived from American cell banks. Cell lines and cancer cell lines (more than 3,000 types), providing recovery and cryopreservation instructions for cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions, etc. Information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
/
The company specializes in providing normal cells from American cell banks. lines, tumor cell lines, cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions, etc. recovery and Cryopreservation instructions information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines from American cell banks, Tumor cell lines and cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions, etc. recovery and cryopreservation Instructions for information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
hCMEC/D3 immortalized human brain microvascular endothelial cells