Normal colon epithelial cells
(Normal colon epithelial cells)
[Cell number]
[Cell abbreviation]
Cells
[Cell name]
(Normal colon epithelial cells)
[Background information]
See cell instructions
[Cell source]
The cells are mainly derived from normal colon epithelial cells
and from a few well-known universities at home and abroad
[Generations]
[Specifications]
Resuscitation culture bottles ( One bottle) or cryopreserved vials (two)
[Number of cells]
*
[Biosafety level]
【Organism】
Human
【Tissue source】
Colon
【Cell morphology】
Epithelial cells
< br/>【Cell Characteristics】
Adhesion
【Cell Viability】
)
【Cell Detection】
Cells do not contain, Mycoplasma, bacteria, yeast and fungi
[Culture conditions]
See cell instructions for details
[Passaging method]
It is recommended to change the medium every two days< br/>
[Freezing conditions]
For details, please refer to the cell instructions
[Main literature]
Please refer to relevant published articles for details
Operations after receipt Process and Precautions
If the cells are adherent cells and are in a suspended or partially suspended state when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to a new culture dish/bottle Continue to culture for another day and replace the remaining adherent cells in the original culture flask with complete culture medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or according to the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
Normal colon epithelial cells grow unevenly If adherent cells are unevenly distributed, they will form islands To grow like this, the cells can be digested, resuspended and dispersed, and then added with fresh medium for culture
Routine cell culture and passage procedures (please strictly follow aseptic operation)
Aspirate the culture in the original culture bottle Base, rinse the cells twice with buffer, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it)
Observe the digestion situation under the microscope, when the edge of the cell shrinks and the adhesion becomes loose (do not It is recommended to digest until the cells float), remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, Add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in culture medium value, change the medium regularly (weekly), and repeat the operation after the cell density reaches % or Cryopreservation
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, the company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, Organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to experiment No more worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell line organisms, growth characteristics, sources, organs, Type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, making your experiments more trouble-free. Annoyance
Normal colon epithelial cells
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, and growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line organisms , growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture services. Technical support allows you to experiment without any worries
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. Provide cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide Comprehensive technical support for cell culture, allowing you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. ), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture. It can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. Provide cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide Comprehensive technical support for cell culture, allowing you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture All-round technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture Comprehensive technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. Provide cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide Comprehensive technical support for cell culture, allowing you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture All-round technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture All-round technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell lines Organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide comprehensive cell culture services. With comprehensive technical support, your experiments will be trouble-free
Normal colon epithelial cells
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 cells) from American cell banks. species), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture , can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing Cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide cells Develop a full range of technical support to make your experiments hassle-free
NCM460 normal colon epithelial cells