Osteocyte
(Bone cell)
[Cell number]
[Cell abbreviation]
Cell
[Cell name] 】
(Bone cells)
[Background information]
See cell instructions
[Cell source]
The main source of cells and a few domestic Established as a department in a famous foreign university
[Generation]
[Specifications]
Resuscitation culture bottle (one bottle) or bone cells
Cryopreservation Tubes (two)
[Number of cells]
*
[Biosafety level]
[Organism]
Mouse
[Tissue source]
Preosseous osteoblasts
[Cell morphology]
Fibroblast-like
【Cell Characteristics】
Adhesion
【Cell Viability】
)
【Cell Detection】
The cells do not contain, mycoplasma , bacteria, yeast and fungi
[Culture conditions]
See cell instructions for details
[Passaging method]
It is recommended to change the medium once every two days
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[Freezing Conditions]
Please refer to the cell instructions for details
[Main Documents]
Please refer to relevant published articles for details
Operation procedures after receipt Notes
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to a new culture dish/bottle to continue. At the same time, the remaining adherent cells in the original culture flask were replaced with complete culture medium containing % serum for the first day of culture. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , or based on the cell growth density, trypsin digestion can be considered and then transferred to a new culture bottle to continue culturing
. Uneven growth. If adherent cells are unevenly distributed, bone cells will grow into islands
, the cells can be digested, resuspended and dispersed, and fresh culture medium added for culture
Routine cell culture and passage procedures (please strictly follow aseptic operation)
Aspirate the culture medium in the original culture bottle, Rinse the cells twice with buffer, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it within 10%). Observe the digestion situation under the microscope. When the edges of the cells shrink and the adhesion becomes loose (digestion is not recommended) until the cells are floating), remove the trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle, add appropriate Complete culture medium, beat the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in the culture medium value, change the medium regularly (weekly), and repeat the operation or freeze it after the cell density reaches %
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Bone cells
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. , providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can Provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks. ), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture. It can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cells strain organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide cell culture All-round technical support allows you to experiment without any worries
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, and provides cell line organisms and growth characteristics , source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture. Let your experiments be hassle-free
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, and provides cell line organisms and growth characteristics , source, organ, type, morphology, culture conditions, application, line, disease, tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture. Let your experiments be trouble-free
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, and provides cell line organisms, growth characteristics, and sources. , organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, allowing you to Experiments are no longer troublesome
Bone cells
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support allows you to experiment without any worries
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, and provides cell line biology body, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture Technical support allows you to experiment without any worries
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from American cell banks, providing cell lines, organisms, Growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture and can provide a full range of cell culture technologies. Support, allowing you to experiment without any trouble
MLO-Y4 bone cells