Human nasal mucosal epithelial cells
(Human nasal mucosal epithelial cells)
[Cell number]
[Cell abbreviation]
Cells
< br/>[Cell name]
(Human nasal mucosal epithelial cells)
[Background information]
See cell instructions
[Cell source] < br/>The main source of cells and a few well-known domestic and foreign universities established departments
[Generation]
[Specifications]
Resuscitation culture bottle (one bottle) or frozen Cryotubes (two)
[Number of cells]
*
[Biosafety level]
[Organism]
Human
[Tissue source]
Nasal mucosa
[Cell morphology]
Human nasal mucosa epithelial cells
Epithelial cells
【Cell Characteristics】
Adhesion
【Cell Viability】
)
【Cell Detection】
Cells do not contain mycoplasma, bacteria, yeast and fungi
[Culture conditions]
See cell instructions for details
[Passaging method]
Two recommended Change the medium once a day
[Freezing conditions]
See cell instructions for details
[Main literature]
Please refer to relevant published articles for details
Operation procedures and precautions after receipt
If the cells are adherent cells and are suspended or partially suspended when received, please centrifuge the suspended cells immediately and add % serum complete culture medium to new cells. The culture dish/bottle continues to be cultured for another day while the remaining adherent cells in the original culture bottle are replaced with complete culture medium containing % serum for another day. After a few days, if the cells in the original bottle or the new bottle do not increase in value but continue to fall off and die, please contact the laboratory technician in time and they will follow up and solve the problem
. Adherent cells grow slowly and increase the serum concentration appropriately (the maximum cannot exceed %) , human nasal mucosal epithelial cells
. If the adherent cells grow unevenly and are unevenly distributed, they will become To grow in an island shape, the cells can be digested, resuspended and dispersed, and then added with fresh medium for culture
Routine cell culture and passage procedures (please strictly follow aseptic operation)
Aspirate out the original culture bottle Rinse the cells twice with culture medium and buffer, add .% trypsin to digest the cells (pay attention to the digestion time, usually control it at)
Observe the digestion situation under the microscope, and when the edges of the cells shrink and the adhesion loosens ( It is not recommended to digest until the cells are floating.) Remove trypsin, add complete culture medium, gently pipet the cell layer, try to blow off the cell layer, blow it loose
, transfer part of the cell suspension to a new culture dish/bottle , add appropriate complete culture medium, mix the cell suspension evenly, and culture it in the incubator
Pay attention to the changes in culture medium value, change the medium regularly (weekly), and repeat the operation after the cell density reaches % Or cryopreservation
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank, providing cell lines, organisms, growth Characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide all-round technical support for cell culture. , allowing you to experiment without any trouble
Human nasal mucosal epithelial cells
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three thousand Various), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have a wealth of cell line culture Experience, can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank. ), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture. Can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three thousand Various), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have a wealth of cell line culture Experience, can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank. ), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture. Can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three thousand Various), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have a wealth of cell line culture Experience, can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
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The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines from American cell banks ( More than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have a wealth of cell With experience in cell culture, we can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three thousand) from American cell banks. Various), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have a wealth of cell line culture Experience, can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the American cell bank. ), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions. We have rich experience in cell line culture. Can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
The company specializes in providing normal cell lines, tumor cell lines, and cancer cell lines (three thousand Various), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, cryopreservation conditions and other recovery and cryopreservation instructions information. We have a wealth of cell line culture Experience, can provide a full range of technical support for cell culture, so that your experiments will no longer be troublesome
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Human nasal mucosal epithelial cells
, the company professionally provides The American cell bank sources normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types), providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, tissues, Cryopreservation conditions and other recovery and cryopreservation instructions information, we have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
, Company Specializing in providing normal cell lines, tumor cell lines, and cancer cell lines (more than 3,000 types) from the U.S. cell bank, providing cell line organisms, growth characteristics, sources, organs, types, morphology, culture conditions, applications, lines, diseases, Tissue, cryopreservation conditions and other recovery and cryopreservation instructions information. We have rich experience in cell line culture and can provide a full range of technical support for cell culture, so that your experiments will be hassle-free
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HNEpC human nasal mucosal epithelial cells